Biomarkers in transplantation: Prospective, blinded measurement of predictive value for the flow cytometry crossmatch after negative antiglobulin crossmatch in kidney transplantation

Flow cytometric crossmatch for kidney transplantation.

Serum samples from 49 patients with panel reactive antibodies of greater than 15% and 17 patients who have related donor pairs were collected at the Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital. Crossmatching was performed by three methods, flow cytometry crossmatch (FCXM), the standard National Institutes of Health (NIH), and the antihuman globulin (AHG) microlympho...

[Experimental Application of Whole Blood Flow Cytometry to HLA Crossmatch for Renal Transplantation.].

BACKGROUND The lymphocytes separated from whole blood are used in HLA flow cytometry crossmatch (FCXM) for renal transplantation. In this study, the methodology of whole blood flow cytometry was applied to FCXM, omitting lymphocyte separation step. METHODS In the 20 cases (including positive 5 cases) of T cell FCXM for renal transplantation, the standard assay using the separated mononuclear ...

Successful liver transplantation from crossmatch-positive donors.

Positive virtual crossmatch with negative flow crossmatch results in two cases.

Pre-transplant (Tx) presence of HLA antibodies (HLA-Ab) especially donor specific antibodies (DSA) has been correlated with post-Tx rejection. While crossmatch (XM) is the specific method to identify DSA, logistical reasons prevent performing a prospective XM in all transplants. In such cases DSA as identified by solid-phase assay (SPA) are being used to perform a virtual crossmatch (VXM). We p...

The Role of the Crossmatch in Kidney Transplantation: Past, Present and Future

Immunogenetic characterization of the transplant recipient with crossmatch is used to minimize graft loss by detecting preformed antibodies. Use of increasingly sensitive tests including flow cytometry crossmatch (FCXM) has been accompanied by near elimination of hyperacute rejection. We reviewed associations of crossmatch results with kidney graft outcomes in contemporary practice, and provide...